Electrophoretic separation at pH 6 of a sample of polypeptide 1 (mw 1000) polypeptide 2 (mw 500) and polypeptide 3 (mw 100) would result in which of the following?
(Note: the isoelectric point of each polypeptide occurs at pH 6)
Isoelectric point table
Polypeptide 1 pI=6
Polypeptide 2 pI=2
Polypeptide 3 pI=9
A. P1 will move the farthest
B. P2 will move the farthest
C. P3 will move the farthest
D. None will move.
My thoughts:
to electrophoretically separate, we need a neg. charge and light...
Electrophoretic Separation & Zwitterion
(Note: the isoelectric point of each polypeptide occurs at pH 6)
Isoelectric point table
Polypeptide 1 pI=6
Polypeptide 2 pI=2
Polypeptide 3 pI=9
A. P1 will move the farthest
B. P2 will move the farthest
C. P3 will move the farthest
D. None will move.
My thoughts:
to electrophoretically separate, we need a neg. charge and light...
Electrophoretic Separation & Zwitterion